Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs and bone marrow (BM)-MSCs by gene expressions. Gene expression profiles of SF-MSCs and BM-MSCs after osteogenic, chondrogenic, and adipogenic induction. Among them, type I collagen, osteocalcin, and osteopontin were for osteogenesis; type II collagen and aggrecan were for chondrogenesis; and peroxisome proliferator activated receptor γ 2 (PPAγ2) and adipocyte protein 2 (aP2) were for adipogenesis, respectively. Three SF-MSCs and BM-MSCs samples were performed for this experiment.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs by histological staining and immunofluorescence staining. Histological staining ( A ) and immunofluorescence staining ( B ) of SF-MSCs after osteogenic (Alizarin red S stain and Type I collagen), chondrogenic (Alcain blue stain and Type II collagen), and adipogenic (Oil red O stain and PPAγ2) induction. Green: extracellular matrix and blue: cell nuclei.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Staining, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Sequences of primers used in real-time PCR.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques:

Journal:

Article Title: Runx2, p53 and pRB status as diagnostic parameters for deregulation of osteoblast growth and differentiation in a new pre-chemotherapeutic osteosarcoma cell line (OS1)

doi: 10.1002/jcp.21921

Figure Lengend Snippet: A) Alizarin-red assays were performed to assess mineralization of human fetal osteoblasts (HFOB), OS1 and SAOS-2 cells cultured in triplicate in osteogenic media for 2, 3 and 4 weeks. Quantification of the Alizarin-red assay was based on the pixel intensities of the staining and presented as a proportion of the control in arbitrary units. By Week 2 (2-W), OS1 cells showed initial mineralization (reflecting osteogenic induction) that doubled by Week 3 (3-W) and decelerated by Week 4 (4-W) (ANOVA, p<0.05). A similar trend was observed for HFOB over the same period (ANOVA, P<0.05). For SAOS-2 cells, mineralization accelerated twice as fast as OS1 and HFOB during the same period, with no significant changes during 4-W (ANOVA, P>0.05); B) Immunohistochemistry staining (DAKO LSAB+ system) show that OS1 cells are positive for osteogenic markers (Type-1 collagen, osteonectin and osteocalcin) (bottom panel). Phosphate-buffered solution (PBS) was substituted for primary antibodies as a negative control (top panel) (Original magnification, X20).

Article Snippet: Slides were washed with PBS, incubated in 3% hydrogen peroxide for 5 minutes followed by immuno-staining for 30 min at 37°C with mouse monoclonal antibodies for Collagen type I, Osteocalcin and Osteonectin (Biodesign Inc, USA).

Techniques: Cell Culture, Staining, Immunohistochemistry, Negative Control